RapidFire365‐MS is a mass spectrometry based approach that provides an automated, label free, direct injection assay, using 96‐well and 384‐well plate format, to assess small molecule inhibition of target enzymes and can also be used for enzyme kinetic studies. The RapidFire365‐MS system comprises a conventional Agilent 6550 quadrupole‐time of flight mass spectrometer coupled directly to a very high-throughput robotic sample injection system, providing a sample throughput rate of 1 sample per ~9 seconds. It takes approximately 1.5 hour per 384 samples. Dedicated software enables deconvolution of data and automated integration using Agilent’s Mass Hunter software platform.
Small molecules (inhibition of target enzymes), Enzyme kinetic studies