Rapid Digestion - Promega Trypsin/Lys-C Kit

Guidelines for rapid digestion using the Promega Trypsin/Lys-C Kit for proteomics are found below, or can be downloaded as a pdf.

Guidelines to protect your samples from contamination with keratin

  1. Try to avoid any contact of samples and solutions with dust, skin or hair
  2. Clean your bench
  3. Wear gloves at all times
  4. Trypsin/Lys-C Mix, MS store the Rapid Trypsin protease, Rapid Trypsin Lys-C protease, Resuspension Buffer and Rapid Digest Buffer at –10°C to –30°C. After reconstitution of the protease in Resuspension Buffer, store at –70°C for up to 1 month. Minimize freeze-thaw cycles of all reagents.
  5. Use ultra-pure water for all solutions (MilliQ water)
  6. Rapid Digestion Kit–Trypsin/Lys-C – Product number :VA1061 (The kit contains sufficient reagents to perform up to 100 reactions).
  7. Protein samples (50µl–1ml) should be resuspended in pure water, mildly-buffered solutions (Tris buffers at a concentration of 25mM or lower, pH 7.0–8.0), or those that have been eluted following affinity purification (with organic acid solutions like 0.1% TFA) for best compatibility with the Rapid Trypsin Digestion workflow

Guidelines for sample submission

  1. Provide 10ul of samples in recovery vials* or vials with insert for small volumes for LC-MS/MS analysis.
    *Autosampler vials appropriate for analysis Waters Total Recovery (part number: 186000385C.)
  2. Label your tube with the sample ID.
  3. Fill in the proteomic submission form to provide us with more information about your sample.