Metabolomics of 2-HG treated LN18 wild-type cells

These data were produced from the analysis of LN18 wild-type (WT) cells, treated with 10 mM 2-hydroxyglutarate (2-HG) or vehicle control for 24 hours. The data were acquired as part of the project “Investigating Isocitrate Dehydrogenase Mutations in Cancer Using New Metabolomics Methods” using multiple liquid chromatography-mass spectrometry methods. Anion-exchange chromatography-mass spectrometry (IC-MS) data were acquired on a Dionex IC-5000+ ion chromatography system hyphenated to a Thermo Fisher Scientific Exactive. Reversed-phase chromatography-mass spectrometry (RP-MS) data were acquired on a Dionex U3000 UHPLC system hyphenated to a Thermo Fisher Scientific Exactive. Derivatised RP-MS data were acquired on a Dionex U3000 UHPLC system hyphenated to a Thermo Fisher Scientific Exactive with sample derivatisation using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate.
Data are .zip compressed to reduce file size. Data files in the .RAW format require Thermo Fisher Scientific Xcalibur software to analyse.