Instrument description:
The UPC2 uses compressed CO2 as the primary mobile phase to leverage the chromatographic principles and selectivity of normal-phase LC while providing the ease-of-use and method development simplicity of reversed-phase LC. The miscibility of CO2 with a wide range of polar and non-polar organic solvents has made the liquid CO2-based mobile phase versatile enough to separate a much wider range of compounds than reversed-phase chromatography, especially for mixtures containing very hydrophobic and/or polar compounds. Not only can CO2-based mobile phases be used with both polar and non-polar stationary phases, but the chromatography can be influenced by modulating solvent gradients with a much wider choice of columns (including chiral columns) using the same mass spectrometry- compatible co-solvents.
Typical applications:
Lipidomics, Analysis of fatty acids
